Figure 5
Microtubule nucleation. (A) Wild-type (WT) and mutant cells (Tax 18 and Tax 11-6) were grown 2 d in the absence of paclitaxel, transfected with EB1-GFP, and viewed at 5 s intervals. Comets representing the plus-ends of newly nucleated microtubules in the area around the centrosome are shown. Scale bar equals 2 μm. (B) Comets newly appearing within a 30 μm2 circular area around the centrosome were counted and averaged from 25 successive images in each of 3-5 separate cells. The y-axis represents the average number of comets per frame. Error bars represent the standard deviation from the mean.
